br Fig Flow cytometry profiles of A cell line after
Fig. 11. Flow cytometry profiles of A549 cell line after 3 h of incubation with biotin-C6-NLCs and C6-NLCs.
Fig. 12. Fluorescence microscopic images of A549 cells after 3 h incubation with biotin-C6-NLCs and C6-NLCs.
confirmed efficient cellular uptake of biotin-NLCs. As it can be seen in Fig. 12, the fluorescent intensity of biotin-NLCs related to the C6 that entered into cells is more than that of the non-targeted NLCs.
In present study, we developed biotin functionalized NLCs for the SUN delivery. B-SA conjugate was synthesized and confirmed by FTIR and H NMR. The formulation variables were optimized using Design Expert Software. The optimized biotin-SUN-NLCs showed acceptable particle sizes with narrow size distributions. Besides, biotin-SUN-NLCs
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showed significantly higher cytotoxic effect in lung cancer A549 cells overexpressing biotin receptor compared to that of non-targeted NLCs and free SUN. The improvement of cellular uptake of biotin-SUN-NLCs into A549 cells was also demonstrated. Taken together, biotin-SUN-NLCs can be used as an efficient targeted chemotherapy to cure a number of cancers overexpressing biotin receptors, including lung cancer. However, further study is required to confirm the therapeutic potential of biotin-SUN-NLCs in cancer therapy in vivo.
Declaration of conflicting interests
The authors report no conflicts of interest.
The authors wish to thank the Research Vice Chancellery of Isfahan University of Medical Sciences for supporting this work.
A biodegradable perivascular wrap for controlled, local and directed drug delivery,
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